Session Index

To image living organisms, optical sectioning and high-speed image acquisition are required. Using diffractive optical elements, such as volume hologram and metalens, we designed and developed a compact diffractive light sheet fluorescence microscope (DLSFM) system and experimentally demonstrated in-vivo fluorescence imaging of Caenorhabditis elegans (C. elegans).

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We explored the cellular localization of μ-opioid (MOP) and nociceptin/orphanin FQ (NOP) receptors regulated by opioids. We overexpressed the fluorescence-tagged receptors (MOP-CFP & NOP-YFP) via transfecting HEK 293 cells transiently and then measured the variations of fluorescent signals in single-particle tracking (SPT) and fluorescence resonance energy transfer (FRET) by two-photon fluorescence microscopy. The results could be helpful in unraveling the cellular signaling after these receptors are stimulated by opioids.

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We have investigated the optical system design to realize nonlinear mesoscope for optical virtual biopsy. For simplifying the system and realizing image stitching, we generalize the relay lenses system while maintaining a satisfactory two-photon uniformity of 95% at a scanning angle ±7°. We obtain a stitched leaf image at sub-micron resolution with the field of view of 1.8×1.8mm2, without moving the sample. We also integrate the system into a portable setup, including the delivery of femtosecond pulses with manageable nonlinear spectral conversions. We will discuss the feasibility of our system for ex vivo skin observation in the presentation.

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The main goal of this study is to design two clinical experiments for examining our diffuse reflectance spectroscopy algorithm’s capability in measuring the variation of cytochrome c and cytochrome c oxidase in mitochondria that are linked to tissue vitality. Our findings suggest that our system could be used to noninvasively monitor wound healing, treatment of hair loss, and diabetic foot caring in the future.

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An Arduino based three gas stage-top incubator is designed and developed for biomedical experiment. The Arduino system is used to control three environment conditions: temperature, gas concentration, and humidity. Our system can provide detection of the gas concentration and creates a moderated environment which are useful for biomedical experiments.

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Optical activity related to molecular chirality are significant in the circular dichroism characteristics to determine the configuration of a chiral molecule. We used L-lactic acid (L-LA) and a transparent glue to produce biodegradable films. Adjusting the number of layers comprising L-LA and glue leads to different phase differences, forming L-LA films. Six layers and 0.8% L-LA solution were the optimal conditions to fabricate an film. The circular-polarization experiment showed that the change in the signal intensity was less than 2% at a specific angle of the L-LA film. In summary, circularly polarized light was successfully produced using the L-LA film.

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In this study, the nondestructive blood glucose concentration evaluation of human using near infrared (NIR) spectroscopy measurement is performed. To perform a quantitative analysis, the high power IR LEDs with wavelengths of 850 and 940 nm are used in NIR reflection spectroscopy measurement. Then the calibration model is investigated to predict the blood glucose concentration of the participants from the NIR reflection measurements alone.

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Jaundice is a phenomenon of high serum bilirubin concentration. The prevalence of neonatal jaundice can reach 80% and 21%. The gold standard is still the concentration of bilirubin in blood samples. However, this method cannot be used for continuous bilirubin level monitoring. Also, drawing blood from newborns can be difficult. Traditional bilirubin meters are too expensive and are affected by skin tone. Our team has developed a handheld DRS device for non-invasive screening and monitoring of neonatal bilirubin.

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In the research, molybdenum disulfide is grown on the light-absorbing layer of the silicon-based solar element, combined with the self-designed Serrated Interdigitated Elec-trode. The photoelectric flow measurement was performed on pleural effusion with three different types of lung cancer cell clinical samples through the photo-generated charge carrier transport mechanism

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Currently, optical coherence tomography (OCT) system requires a high-performance desktop which increases the system cost and complexity. In this study, we proposed an OCT system based on a field programmable gate array (FPGA) to replace the high-performance desktop in conventional OCT systems. As a preliminary study, we used an evaluation FPGA board to validate its feasibility to be implemented in the processing framework we have developed using the spectral-domain OCT system. The preliminary results including system framework and comparison of the processing performance with respect to conventional OCT system will be provided as well.

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We present on using polarization-resolved second harmonic generation (P-SHG) microscopy to study changes in the molecular structure of starch granules modified by gamma irradiation. With the characteristic of SHG polarization anisotropy, we extracted the second-order susceptibility, χ(2), tensor of amylopectin, which reflects the underlying molecular details. Then P-SHG microscopy in conjunction with SHG-circular dichroism (CD) were used to identify the inherent chirality of starches and their reaction to different dosages of gamma irradiation. For the first time, the relationship between starch achirality (χ21/χ16 and χ22/χ16) and chirality (χ14/χ16) determining susceptibility tensor ratios have been elucidated.

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Multiphoton excited fluorescence microscopy provides a great spatial excitation resolution for 3D image reconstruction in sub-micron resolution, and is suitable for biological imaging in vivo. We demonstrate a self-built scanning-based MPEFM system. The system lateral resolution is around 0.7 μm and axial resolution is 2.2 μm respectively. We have shown the large scale imaging ability by synchronization with the 3-axis mechanical stage, laser scanning, and fluorescence signal acquisition. The full size of mouse pulmonary fibrosis tissue is successfully reconstructed in high contrast. The experiment result shows the proposed MPEFM system yields a great feasibility for noninvasive biological imaging and analysis.

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As early as mid-19th century, artificial mechanics was proved to change cellular morphology and gene expression. Recent research shows that telogen elongation of mice skin under appropriate tension can stimulate the regeneration of hair follicle. To measure the skin tension required for regeneration, in this study, we used Mueller Matrix polarimetry to record the mouse skin image, while the mouse skin was gradually stretched in eight days. Combining the traditional skin tension measurement via spring and neural network model, the relationship between skin polarization properties and tension was found out. That provides a non-contacting method to measure the skin tension.

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Spruce is the commonly-used tonewood for violin-family instruments. It’s been shown chemical treatment and aging can cause property changes of wood and affect the acoustic properties of instruments. To reveal the spectral and spatial properties of woods, which abundantly contain lignin and cellulose, two-photon hyperspectral microscopy has been introduced. Through the hyperspectral data containing two-photon fluorescence and second-harmonic generation (SHG) information, the spatial content variations of different lignin fluorescent bases and SHG can be obtained microscopically. Comparing woods without process and with aging, heating, and chemical treatments, the effects of different processes have been reveal through the spectral variations.

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